Efficacy of sublingual immunotherapy with Dermatophagoides farinae drops on patients with allergic rhinitis of different symptom severity / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To evaluate the efficacy of sublingual immunotherapy (SLIT) with Dermatophagoides farinae drops for allergic rhinitis (AR) of different symptom severity.@*METHOD@#This retrospective analysis to receive SLIT treatment of 143 cases of patients with allergic rhinitis, according to the severity of disease symptoms divid- ed into two groups, moderate group (62 patients) and severe group (81 patients). Before SLIT and after SLIT for half year, 1 year and 1. 5-2.0 years, the TNSS, TMS and sign scores of patients with allergic rhinitis were evaluated.@*RESULT@#The TNSS, TMS and sign scores had continuously improved significantly after SLIT for half year, 1 year and 1.5-2.0 years in two groups as compared with baseline (P 0.05). Half year after SLIT treatment, in two groups for sign scores, there were significant differences (Z = 3.32, P 0.05) and TMS (Z = 0.37, P > 0.05). 1 and 1.5-2.0 years after SLIT, there were no significant differences in two groups for TNSS, TMS and sign scores (P > 0.05).@*CONCLUSION@#SLIT with Dermatophagoides farinae drops for 1.5-2.0 years is effective in the patients with allergic rhinitis of different symptom severity. And equivalent efficacy could be achieved for different symptom severity.

Low dose of ouabain protects injury of spiral ganglion neurons in vitro / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To evaluate the protective effect of low dose ouabain on injury of cultured spiral ganglion neurons evoked by trophic factors deprived and to explore the mechanism.@*METHOD@#Spiral ganglion neurons were cultured in vitro for 7 days, and then exposed to Neurobasal medium + B27 supplement, Neurobasal medium only or Neurobasal medium + 10 nmol/L ouabain, respectively. After 48 h exposed to different medium, spiral ganglion neurons were stained by FITC labeled Annexin-V and PI, then apoptosis index were calculated using fluorescent microscope and flow cytometry, respectively. In addition, spiral ganglion tissues were cultured for 48 h to evaluate dendrite growth of spiral ganglion neurons in each group. Immunocytochemistry were performed to detect the level of Bcl-2 in each group at 6 h and 12 h.@*RESULT@#Spiral ganglion neurons exposed to Neurobasal medium +10 nmol/L ouabain have a similar apoptosis index compare with that of Neurobasal medium + B27 supplement, but a much lower apoptosis index than that of Neurobasal medium only. In addition, dendrite growth of spiral ganglion neurons exposed to Neurobasal medium +10 nmol/L ouabain was much longer than that of Neurobasal medium only. Bcl-2 level increased in spiral ganglion neurons exposed to Neurobasal medium + 10 nmol/L ouabain at 6 h.@*CONCLUSION@#Low dose of ouabain protects injury of spiral ganglion neurons evoked by trophic factors deprived in vitro. This effect may mediated by increasing the level of Bcl-2.

Epidemic parotitis being accompanied by acute pancreatitis and sensorineural hearing loss: case report and literature review / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To review and explore the clinical characteristics, acoustic features, early diagnosis and prognosis of epidemic parotitis being accompanied by acute pancreatitis and sensorineural hearing loss (SNHL).@*METHOD@#Report and analysis the clinical data of a patient with epidemic parotitis being accompanied by acute pancreatitis and sensorineural hearing loss and review the relevant literatures.@*RESULT@#Epidemic parotitis virus could produce lesion in more than one organ resulting in the corresponding complications. It was uncommon when a patient presented epidemic parotitis, acute pancreatitis and sensorineural hearing loss at the same time. Unilaterally total hearing loss was the most common type of the SNHL, and the prognosis was bad.@*CONCLUSION@#Epidemic parotitis can be accompanied by one or more complication, including SNHL. Auditory brainstem response (ABR) is of great value in early diagnosis of SNHL in patients with epidemic parotitis.